Posts Tagged ‘depopulation’

http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F4647773 Method of continuous production of retroviruses (HTLV-III) from patients with AIDS and pre-AIDS Abstract A cell system is disclosed for the reproducible detection and isolation of human T-lymphotropic retroviruses (HTLV-family) with cytopathic effects (HTLV-III) from patients with the acquired immune deficiency syndrome (AIDS), pre-AIDS and in healthy carriers. One neoplastic aneuploid T-cell line derived from an adult with lymphoid leukemia, and termed HT, was susceptible to infection with the new variants of HTLV, which are transformed and providing T-cell populations which are highly susceptible and permissive from HTLV-III, and convenience for large scale production, isolation and biological detection of the virus. Inventors: Gallo; Robert C. (Bethesda, MD), Popovic; Mikulas (Bethesda, MD) Assignee: The United States of America as represented by the Department of Health (Washington, DC) [*] Notice: The portion of the term of this patent subsequent to May 28, 2002 has been disclaimed. Appl. No.: 06/602,946 Filed: April 23, 1984 Current U.S. Class: 435/239 ; 424/208.1; 435/235.1; 435/372.3; 435/948 Current International Class: C12N 5/06 (20060101); C12N 7/00 (20060101); C12N 007/02 (); C12N 007/00 (); C12N 005/00 (); C12R 001/91 () Field of Search: 435/235,239,240,948,5,29 424/89 128/1T References Cited [Referenced By] U.S. Patent Documents 4464465 August 1984 Lostrom et al. 4520113 May 1985 Gallo et al. Other References Popovic et al, Science, 224(4648):497-500, May 4, 1984. . Gallo et al, “Isolation of Human T-Cell Leukemia Virus in Acquired Immune Deficiency Syndrome (AIDS)”, Science, 220, pp. 865-867 (5-1983). . Barre-Sinoussi et al, “Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for AIDS”, Science, 220, pp. 868-871 (5-1983). . Marx, “Strong New Candidate for AIDS Agent”, Science, 224, pp. 475-477 (5-1984).. Primary Examiner: Warren; Charles F. Assistant Examiner: Tarcza; John Edward Attorney, Agent or Firm: Roberts, Jr.; John S. Claims We claim: 1. A method for continuous production of HTLV-III virus which comprises infecting in cell culture highly susceptible, permissive cells consisting of a neoplastic aneuploid HT-cell line with said virus, said cells preserve the capacity for permanent growth after the infection with said virus, growing said cells under conditions suitable for cell growth and recovering said virus produced by said cells. 2. The method of claim 1, wherein said virus consists of cytopathic variants of HTLV. 3. The method of claim 1 wherein said infecting comprises cocultivating said virus with said cells to produce a cell line and recovering said cell line. 4. The method of claim 1 wherein said infecting comprises cell-free infection of said cells with said virus. 5. The method of claim 1 wherein said cells are neoplastic aneuploid T-cells derived from an adult with lymphoid leukemia. 6. A process for the continuous production of HTLV-III virus which comprises cocultivating said virus with a target HT-cell to produce an infected cell line, growing said cell line and recovering said virus from supernatants produced by said cell line. 7. The process of claim 6 wherein said target T-cell is a neoplastic aneuploid T-cell susceptible to infection with HTLV-III. 8. A process for the continual production of HTLV-III by infecting T-cells in cultures which comprises cocultivating HTLV-III virus with an HT-clone to produce an infected cell line, said clone being an aneuploid T-cell line derived from lymphoid leukemia, growing said cell line and recovering said virus from supernatants produced by said cell line. 9. The process in claim 8 wherein said clone is a mature T-cell phenotype of OKT3.sup.+ (62%), OKT4.sup.+ (39%) and OKT8.sup.-. 10. A method of producing a cell line containing an antigen of HTLV-III which comprises infecting an HT-cell line derived from lymphoid leukemia and susceptible to infection with HTLV-III, said cell line is capable of continuous large-scale production of HTLV-III, and growing the infected cell line under conditions suitable for growth. 11. The method of claim 10 wherein said cell line is a neoplastic aneuploid T-cell line. 12. The method of claim 10 wherein said HTLV-III are variants of human T-lymphotropic retrovirus, exhibit cytopathic effects and are non-transforming. 13. A cell line containing HTLV-III designated H9/HTLV-III.sub.B, ATCC Accession CRL 8543. 14. A process for producing a cell line H9/HTLV-III.sub.B which comprises infecting a target T-cell with HTLV-III virus to produce a cell line and recovering same, said infecting process overcomes the normal cytopathic effect of HTLV-III and preserves the immortal growth capacity of the target cell. 15. An HT cell line permanently infected with HTLV-III virus, wherein said cell line continually produces said virus. Description The present invention describes a cell system for the reproducible detection and isolation of human T-lymphotropic retroviruses (HTLV-family) with cytopathic or cell killing effects (HTLV-III) from patients with the acquired immune deficiency syndrome (AIDS), pre-AIDS and in healthy carriers. One neoplastic aneuploid T-cell line derived from an adult with lymphoid leukemia, and termed HT, was susceptible to infection with the new variants of HTLV, providing T-cell populations which are highly susceptible and permissive for HTLV-III, and convenience for large scale production, isolation, and biological detection of the virus. BACKGROUND OF THE INVENTION The disclosure of this invention is contained in the following journal articles: Gallo et al., “Detection, Isolation, and Continuous Production of Cytopathic Human T-Lymphotropic Retroviruses (HTLV-III) from Patients with AIDS and pre-AIDS,” Science, in press; and Gallo et al., “Human T-Lymphotropic Retrovirus, HTLV-III, Isolated from AIDS Patients and Donors at Risk for AIDS,” in press. Epidemiologic data strongly suggest that acquired immune deficiency syndrome (AIDS) is caused by an infectious agent which is apparently horizontally transmitted by intimate contact or blood products. Though the disease is manifested by opportunistic infections, predominantly Pneumocystis carcinii pneumonia and Kaposi’s sarcoma, the underlying disorder affects the patient’s cell-mediated immunity with absolute lymphopenia and reduced helper T-lymphocyte (OKT4.sup.+) subpopulation(s). Moreover, before a complete clinical manifestation of the disease occurs, its prodrome, pre-AIDS, is frequently characterized by unexplained chronical lymphadenopathy and/or leukopenia involving a helper T cell subset. This leads to the severe immune deficiency of the patient, suggesting that a specific subset of T-cells is the primary target for an infectious agent. Although patients with AIDS or pre-AIDS are often chronically infected with cytomegalovirus or hepatitis B virus, for various reasons these appear to be opportunistic or coincidental infections apparently not linked to the immunological response deficiency. It is believed that the cause of AIDS may be a virus from the family of human T-cell lymphotropic retroviruses (HTLV) which, prior to the present invention, comprised two major well characterized subgroups of human retroviruses, called human T-cell leukemia/lymphoma viruses, HTLV-I and HTLV-II. The most common isolate, HTLV-I, is mainly obtained from patients with mature T-cell malignancies. Seroepidemiological studies, in vitro biological effects, and nucleic acid hybridization data indicate that HTLV-I is etiologically associated with these malignancies, affecting adults primarily in the south of Japan, the Caribbean and Africa. HTLV of subgroup II (HTLV-II) was first isolated from a patient with a T-cell variant of hairy cell leukemia. To date, this is the only reported isolate of HTLV-II from a patient with a neoplastic disease. Virus isolation and seroepidemiological data show that HTLV of both subgroups can sometimes be found in patients with AIDS. Evidence suggests that the retrovirus(es) of the HTLV family is an etiological agent of AIDS based on the following: (1) there is precedence for an animal retrovirus cause of immune deficiency (feline leukemia virus in cats); (2) retroviruses of the HTLV family are T-cell tropic; (3) they preferentially infect “helper” T-cells (OKT4.sup.+); (4) they have cytopathic effects on various human and mammalian cells as demonstrated by their induction of cell syncytia formation; (5) they can alter some T-cell functions; (6) in some cases infection may result in selective T-cell killing; and (7) they are transmitted by intimate contact or through blood products. The presence of antibodies directed to cell membrane antigens of HTLV infected cells has been shown in sera of more than 40% of patients with AIDS [Essex et al., Science, 220:859 (1983)]. This antigen has since been defined as part of the envelope of HTLV [Schupbach, et al., Science, in press; and Lee, et al., Proc. Nat. Acad. Sci. U.S.A., in press]. The original detection and isolation of the various HTLV isolates were made possible by two earlier developments: the discovery of T-cell growth factor (TCGF), also called Interleukin 2 (Il-2), which enabled the routine selective growth of different subsets of normal and neoplastic mature T-cells [Ruscetti, et al., J. Immunol., 119:131 (1977); and Poiesz, et al., Proc. Nat. Acad. Sci. U.S.A, 77:6134 (1980)] and the development of sensitive assays for detection of retroviruses based on reverse transcriptase assays. The methods of HTLV isolation and transmission involved a cocultivation procedure using permissive T-cells for the virus. The use of normal human T-cells in cocultivation experiments preferentially yielded HTLV of both subgroups with immortalizing (transforming) capability for some of the target T-cells. However, HTLV variants (now termed HTLV-III), lack immortalizing properties for normal T-cells and mainly exhibit cytopathic effects on the T-cells and is now believed to be the cause of AIDS. In fact, such variants were frequently but only transiently detected using these normal T-cells as targets in cocultivation or cell-free transmission experiments. The cytopathic effect was overcome by finding a highly susceptible, permissive cell for cytopathic variants of HTLV, thus preserving the capacity for permanent growth after infection with the virus. The present invention discloses the identification and characterization of this new immortalized T-cell population and its use in the isolation and continuous high- level production of such viruses from patients with AIDS and pre-AIDS. Early experiments identified one neoplastic aneuploid T-cell line, termed HT, derived from an adult with lymphoid leukemia, that was susceptible to infection with the new cytopathic virus isolates. This cell line is a sensitive target for transmission of these virus isolates (HTLV-III) and it allows continuous large-scale virus production and development of specific immunologic reagents and nucleic acid probes useful for comparison of these new isolates among themselves and with HTLV-I and HTLV-II. In addition to their differences in biological effects that distinguish them from HTLV-I and HTLV-II, HTLV-III also differs from these known HTLV subgroups in several immunological assays and in morphology. However, these new retroviruses are T4 lymphotropic and exhibit many properties similar to HTLV-I and II, including similar properties of the reverse transcriptase, cross reactivity of structural proteins as determined by heterologous competition radioimmune assays with patients’ sera and with animal hyperimmune sera, and induction of syncytia. These new retrovirus isolates are collectively designated HTLV-III, together with detectable differences in some of their proteins and genetic information, HTLV-III’s ability to kill T-cells clearly separates these variants from other members of the HTLV family. Read the full patent via the US patent website

Hidden Agendas

 

Uploaded by wrxstiable on Jul 27, 2009

This is true and its happening now. Please open your eyes people before we have no rights left to oppose. Save our next generation from being slaves. Global warming is a lie , It is part of agenda 21 of UN. UN will control the future world and soon humanity would be put on trial for ruining the earth and not preserving it for the generations to come. This is the mother of scams. Media is our enemy. All big names like cnn, fox, cnbc, bbc and many more have signed a contract with UN to keep their secrets and be their alloys in this so called “earth saving mission”.

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This video uses copyrighted material in a manner that does not require approval of the copyright holder. It is a fair use under copyright law.Copyright Disclaimer Under Section…

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Truth11

Wednesday, August 29, 2012

Susanne Posel, Contributor
Activist Post

DeCODE Genetics (DCG) has published a study that states Icelandic fathers who are in their 40s pass down more genetic mutations than their 20 year-old counterparts. According the DCG, 97% of genetic mutations are derived from older fathers.

Because males produce sperm throughout their lives, and the genetic make-up of that sperm is created from mutations based on previously produced sperm. This study supposes that increase in autism could be connected to these genetic mutations.

This has led to an increase in genetic defects, suggests lead researcher Dr. Kari Stefansson. She asserts:

Society has been very focused on the age of the mother. But apart from [Down’s Syndrome] it seems that disorders such as schizophrenia and autism are influenced by the age of the father and not the mother.

Studies into DNA with regard to reproduction and children harken back…

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PN

by James Corbett
BoilingFrogsPost.com

August 7, 2012

Beyond even the medical debate over the benefits of fluoride, or an examination of the history and sponsorship of this process, there is a deeper debate taking place. This debate is not over whether or not fluoride is safe or effective, but whether the government should be adding it to the water supply in the first place.

As many argue, this practice amounts to forced medication of the population without their consent, and without regard to the case history or medical circumstances of any of the “patients” (i.e. the general public) who are exposed to this “medication.”

CONTINUE WATCHING: http://ur1.ca/9wsty
TRANSCRIPT AND SOURCES: http://www.corbettreport.com/?p=5327

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By Dr. Mercola

african child Poor African Countries Get Vaccines but No Food or Clean Water

As one of the world’s most well-known and respected voices, Microsoft founder Bill Gates has a unique opportunity to call attention to important social issues and make a huge impact worldwide.

Unfortunately Gates, through his foundation, has been partnering with not only biotech giant Monsanto to hoist genetically modified seeds on third-world countries, but also with Big Pharma, to whom he pledged $10 billion to provide vaccinations to children around the world.

This is billed as a humanitarian effort to save lives, but what children in developing countries need is healthy food, clean water and better sanitation.

These are the keys to preventing the spread of infectious disease, and they are being wholly ignored by the likes of Bill Gates and other vaccine proponents – at the children’s expense!

The Aftermath of a Bill Gates Vaccine Campaign …

An American family, the Gianelloni’s, visited a village in Uganda shortly after a Bill Gates vaccine campaign swept through and discovered what Bill Gates’ money does for hungry, sick children – essentially nothing.

The family found that the children were starving, living on one meager meal a day. Their only water source was the same stagnant stream that they bathed in. They had no sewage or sanitation. But, thanks to Gates, they were now vaccinated against measles and polio. Never mind that the most pressing epidemics in the area were actually Yellow Fever, malaria, HIV/AIDs and diarrhea …

Worse yet, one little girl who had received a measles vaccine two weeks earlier was now suffering with the measles as a result! After this blogger left, thanks to her and the mission group that arrived with her, the village had a water tank and clean water system, a cow, and a year’s worth of rice and beans. You can probably understand why the blogger made this comment about Gates’ “philanthropy”:

“I don’t care who you are or what side of the vaccine philosophy you fall under, there is no logic in the world that can explain that going into a remote village and giving children who only eat one meal a day and have never had clean drinking water, a vaccine. 

Seriously? Think about it. Can you imagine walking up to this precious little girl and saying ”I know you are starving, but here is a measles vaccine instead. I promise this will make you much healthier than food or water”. It’s a scary day when simple logic no longer exists.

Food & Water, nope. Vaccines, yep. And innocent children suffer the consequences. It’s absurd.

Food, Water, Sanitation is What’s Needed to Help Prevent Disease

The most vulnerable of the world’s children are those in the poorest countries where death and disease are often a result of malnutrition and lack of adequate sanitation and clean drinking water. In many third-world countries, children are often battling some sort of infection 200 days out of the year. Vaccines can be devastating to these already immunosuppressed children, as well as to adults, because vaccines often weaken and confuse the immune system, which ultimately increases the recipient’s susceptibility to the very infectious diseases vaccines are designed to prevent.

Nonetheless, emerging vaccine markets like third-world countries will soon outgrow developed markets by hundreds of billions of dollars. Emerging markets are areas of the world that are beginning to show promise as a profitable venture for many products, including vaccines. And emerging markets – primarily in developing countries in Southeast and Central Asia, and Africa – have been on vaccine makers’ radar for quite some time.

One reason that vaccine makers are interested in these parts of the world is that that’s where most of the world’s deaths from major infectious diseases occur. The only problem has been that, until recently, making vaccines for undeveloped countries with no money to pay for them was not exactly a profitable goal for vaccine makers.

Concerned that developed countries would have little or no resources for addressing serious infectious diseases if vaccine makers continued their pull-out, the World Health Organization and the G8 – the top developed countries in the world – responded with a plan for enticing vaccine companies to stay in the business. That plan was called Advance Market Commitments (AMCs).

AMCs Guarantee Drug Company Profits

Under AMCs, developed countries make legal, binding agreements to purchase vaccines that are needed in low-income countries. The purchase guarantees a bottom line for the manufacturers. In return, the manufacturers promise to sell those vaccines at reduced prices in the countries where they are most needed.

The idea is simple: “rich” nations sign legally binding commitments to purchase and/or finance an AMC vaccine once it’s ready for market. In return for the guaranteed market and income, drug companies promise to sell the new vaccine to “poor” countries at vastly reduced prices.

To speed up the process, the World Health Organization “prequalifies” AMC vaccines in an approval process that slices years off the time it normally takes a vaccine to make it to market.

Unfortunately, legally binding, advance market commitments to purchase vaccines that are mostly needed in third-world countries could backfire on developed countries that don’t need – or want – certain vaccines.

For instance, HPV (human papillomavirus) statistics show that HPV causes 4,000 deaths from cervical cancer per year in the United States, compared to 274,000 worldwide, 88 percent of which are in developing countries. So why were the HPV vaccines Gardasil and Cervarix — which have known safety issues — introduced in the U.S. and Europe, first, instead of going straight to where they’re needed most, if not to help sell huge quantities of the vaccine at premium prices, in anticipation of it becoming an AMC?

Even Gates and a Leading Vaccine Maker Admit Clean Water is Key

Malaria is another one of the top neglected diseases that world health leaders want to address with AMCs, but the ability to resist diseases like malaria requires a strong immune system, and for that, you require good nutrition, clean drinking water, and sanitation. If we want to help people in other countries to lower their malaria rates, and rates of other infectious diseases (like infection-associated diarrhea, which is one of the most common, and most preventable causes of death among children in the developing world) it would be wise to focus on these basics first.

Infectious organisms are more likely to penetrate the bodies of malnourished children due to inadequate vitamin C, which causes their skin to break down more easily and facilitates the entry of bacteria and other pathogens. The same is true for vitamin A deficiency, another common third-world problem, which results in increased susceptibility to infection and which could be rectified in individuals for pennies a day. Also, the living conditions of third-world children are often so poor that they are exposed to inordinately large numbers of pathogens, from which they have little defense.

In order to eradicate infectious disease from a nation, you also have to first address compromised immune systems. If you hit immune suppressed children with a potent, adjuvant-loaded vaccine, you’re far more likely to create new disease, not eradicate it.

With all of the billions being poured into vaccines to “save” the children, how many water purification systems could have been built? How many sanitation facilities? How many rations of meat and fresh produce?

Even Bill Gates himself has admitted that vaccines alone don’t eradicate disease. In a Wall Street Journal article about the resurgence of polio in African countries, Gates said that’s why he is revamping his disease fight to incorporate health, hygiene, and clean drinking water programs into vaccination programs.1 Polio spreads, after all, largely through feces-contaminated water, so ignoring that major risk factor while trying to eradicate the disease is ignorant, to put it nicely.

What’s really interesting is that at least one major vaccine maker has also echoed these sentiments, as evidenced on the front page of GlaxoSmithKline’s presentation to shareholders in June 2010:2

“With the exception of clean drinking water, vaccines are the most cost-effective public health measure,” GSK said.

What if, just what if, the same amount of money that has been spent on vaccines over the past decade had been spent on sanitation facilities, toilets, healthy food and clean water instead?

What You Can Do to Make a Difference

Increasing numbers of vaccines are being introduced not only in third-world countries but also in the developed world, and it’s simply not wise to blindly depend on the information coming directly from the vaccine makers’ PR departments, or from federal health officials, agencies or foundations that are mired in conflicts of interest with industry …

No matter what vaccination choices you make for yourself or your family, it is a basic human right to be fully informed about all the risks of medical interventions and pharmaceutical products, like vaccines, and have the freedom to refuse if you conclude the benefits do not outweigh the risks for you or your child.

Unfortunately, the business partnership between government health agencies and vaccine manufacturers is too close and is getting out of hand. There is a lot of discrimination against Americans, who want to be free to exercise their human right to informed consent when it comes to making voluntary decisions about which vaccines they and their children use.

We cannot allow that to continue.

While it seems “old-fashioned,” the only truly effective actions you can take to protect the right to informed consent to vaccination and expand your rights under the law to make voluntary vaccine choices, is to get personally involved with your state legislators and the leaders in your community.

THINK GLOBALLY, ACT LOCALLY.

Mass vaccination policies are made at the federal level but vaccine laws are made at the state level, and it is at the state level where your action to protect your vaccine choice rights will have the greatest impact.

Signing up to be a user of NVIC’s free online Advocacy Portal at www.NVICAdvocacy.org gives you access to practical, useful information to help you become an effective vaccine choice advocate in your own community. You will get real-time Action Alerts about what you can do if there are threats to vaccine exemptions in your state. With the click of a mouse or one touch on a Smartphone screen you will be put in touch with YOUR elected representatives so you can let them know how you feel and what you want them to do. Plus, when national vaccine issues come up, you will have all the information you need to make sure your voice is heard. So please, as your first step, sign up for the NVIC Advocacy Portal.

Right now, in California, the personal belief exemption is under attack by Pharma-funded medical trade organizations and public health officials trying to get a bill (AB 2109) passed that would require parents to get a medical doctor’s signature to file an exemption for personal religious and conscientious beliefs. Watch NVIC’s 90-second public service message and learn more about what you can do if you are a California resident.

Internet Resources

To learn more about vaccines, I encourage you to visit the following web pages on the National Vaccine Information Center (NVIC) website at www.NVIC.org:

  • NVIC Memorial for Vaccine Victims: View descriptions and photos of children and adults, who have suffered vaccine reactions, injuries and deaths. If you or your child experiences an adverse vaccine event, please consider posting and sharing your story here.
  • If You Vaccinate, Ask 8 Questions: Learn how to recognize vaccine reaction symptoms and prevent vaccine injuries.
  • Vaccine Freedom Wall: View or post descriptions of harassment by doctors or state officials for making independent vaccine choices.
  • Vaccine Ingredient Calculator (VIC): Find out just how much aluminum, mercury and other ingredients are in the vaccines your doctor is recommending for you or your child.
  • Vaccine Adverse Events Reporting System (VAERS) on MedAlerts. Search the government’s VAERS database to find out what kinds of vaccine reactions, injuries and deaths have been reported by patients and heath care workers giving vaccines.

Find a Doctor Who Will Listen to Your Concerns

Last but not least, if your pediatrician or doctor refuses to provide medical care to you or your child unless you agree to get vaccines you don’t want, I strongly encourage you to have the courage to find another doctor. Harassment, intimidation, and refusal of medical care is becoming the modus operandi of the medical establishment in an effort to punish those patients and parents, who become truly educated about health and vaccination and want to make vaccine choices instead of being forced to follow risky one-size-fits-all vaccine policies.

If you are treated with disrespect or are harassed in any way by a doctor (or government official), do not engage in an unproductive argument. You may want to contact an attorney, your elected state representatives or local media, if you or your child are threatened.

That said, there is hope.

At least 15 percent of young doctors recently polled admit that they’re starting to adopt a more individualized approach to vaccinations in direct response to the vaccine safety concerns of parents. It is good news that there is a growing number of smart young doctors, who prefer to work as partners with parents in making personalized vaccine decisions for children, including delaying vaccinations or giving children fewer vaccines on the same day or continuing to provide medical care for those families, who decline use of one or more vaccines.

So take the time to locate and connect with a doctor who treats you with compassion and respect and is willing to work with you to do what is right for your child, and isn’t just competing for government incentives designed to increase vaccination rates at any cost.

Read the entire article here: http://articles.mercola.com/sites/articles/archive/2012/08/06/healthy-foods-not-vaccines.aspx

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